Pengaruh suhu dan lama penyimpanan terhadap antioksidan ASI

  • Enggar Akademi Kebidanan Palu Sulawesi Tengah
  • Margareta Paula Klara Akademi Kebidanan Palu Sulawesi Tengah


Background: Breast milk contains antioxidants that can inhibit oxidative stress in infants. Milking is now a much-done option. Breast milk is stored so that the baby can drink it at any time. The presence of temperature treatment and long storage of breast milk triggers physical and chemical changes, one of which is antioxidants that babies desperately need to combat the effects of free radicals.
Objective: The research aimed at comparing antioxidants in the fresh breast milk and storage breast milk.
Methods: This was the true experimental research. Samples were taken using the purposive sampling technique of 24 samples. The breast milk was tested its antioxidant content using DPPH, initial control, and giving two treatment factors. The first treatment was the breast milk storage at room temperature (fresh breast milk), refrigerator temperature, and freezer temperature. The second treatment was breast milk storage for 7, 14, 30, and 60 days. The data were analyzed using T-Test and repeated Anova.
Results: The research result indicates that there is a decrease of the antioxidants between the fresh breast milk and the breast milk stored in the refrigerator temperature of 20C with the storage duration of 7 days, and freezer temperature of -160C with the storage duration 14, 30, and 60 days, p=0.000.
Conclusion: The antioxidant is better stored at the temperature of -160C than the temperature of 20C. The longer the storage, the lower the breast milk antioxidant.

Author Biographies

Enggar, Akademi Kebidanan Palu Sulawesi Tengah

Jalan Cendrawasih No. 44 Palu, Sulawesi Tengah

Margareta Paula Klara, Akademi Kebidanan Palu Sulawesi Tengah

Jalan Cendrawasih No. 44 Palu, Sulawesi Tengah


Organization, U. N. C. F. and W. H. Guideline: updates on HIV and infant feeding: the duration of breastfeeding, and support from health services to improve feeding practices among mothers living with HIV. Geneva World Heal. Organ. (2016).

Kemenkes RI. Rencana Strategis Kementrian Kesehatan Tahun 2015-2019. Kementerian Kesehatan RI (2015).

Buonocore, G., Perrone, S. & Tataranno, M. L. Oxidative stress in the newborn. Oxid. Med. Cell. Longev. 2017, 2–4 (2017).

Perrone, S. et al. Effects of lutein on oxidative stress in the term newborn: A pilot study. Neonatology 97, 36–40 (2009).

Perrone, S., Tataranno, M. L., Stazzoni, G. & Buonocore, G. Biomarkers of oxidative stress in fetal and neonatal diseases. J. Matern. Neonatal Med. 25, 2575–2578 (2012).

Yao, L., Friel, J. K., Suh, M. & Diehl-Jones, W. L. Antioxidant properties of breast milk in a novel in vitro digestion/enterocyte model. J. Pediatr. Gastroenterol. Nutr. 50, 670–676 (2010).

Oveisi, M. R. et al. Human breast milk provides better antioxidant capacity than infant formula. Iran. J. Pharm. Res. 9, 445–449 (2010).

Yuksel, S., Yigit, A. A., Cinar, M., Atmaca, N. & Onaran, Y. Oxidant and antioxidant status of human breast milk during lactation period. Dairy Sci. Technol. 95, 295–302 (2015).

Molyneux, P. The Use of the Stable Free Radical Diphenylpicryl-hydrazyl (DPPH) for Estimating Antioxidant Activity. Songklanakarin J. Sci. Technol. 26, 211–219 (2004).

Aksu, T. et al. The effects of breast milk storage and freezing procedure on interleukine-10 levels and total antioxidant activity. J. Matern. Neonatal Med. 28, 1799–1802 (2015).

Xavier, A. M., Rai, K. & Hegde, A. M. Total antioxidant concentrations of breastmilk-an eye-opener to the negligent. J. Heal. Popul. Nutr. 29, 605–611 (2011).

Sari, F. N. et al. Antioxidant capacity of fresh and stored breast milk: Is -80°C optimal temperature for freeze storage. J. Matern. Neonatal Med. 25, 777–782 (2012).

Akdag, A. et al. Storage at -80°C Preserves the Antioxidant Capacity of Preterm Human Milk. J. Clin. Lab. Anal. 28, 415–418 (2014).

Silvestre, D. et al. Frozen breast milk at -20°C and -80°C: A longitudinal study of glutathione peroxidase activity and malondialdehyde concentration. J. Hum. Lact. 26, 35–41 (2010).

Marinković, V. et al. Antioxidative activity of colostrum and human milk: Effects of pasteurization and storage. J. Pediatr. Gastroenterol. Nutr. 62, 901–906 (2016).

Chang, Y. C., Chen, C. H. & Lin, M. C. The macronutrients in human milk change after storage in various containers. Pediatr. Neonatol. 53, 205–209 (2012).

Ruhana, A., Istiqomah, N. N. & Prijadi, B. Pengaruh Waktu dan Suhu persiapan Terhadap Kadar Asam Amino Taurin pada ASI. Indones. J. Hum. Nutr. 3, 60–68 (2016).

Aryani, F. S. U. S. T. Jurnal Keperawatan Intan Husada, Vol.4 No.1, Januari 2017 1. Stud. Pengaruh Suhu Penyimpanan Terhadap Kadar Asam Lemak Omega-3 Pada Air Susu Ibu 4, 1–10 (2017).

Ramírez-Santana, C. et al. Effects of cooling and freezing storage on the stability of bioactive factors in human colostrum. J. Dairy Sci. 95, 2319–2325 (2012).

Chang, J. C. et al. Influence of prolonged storage process, pasteurization, and heat treatment on biologically-active human milk proteins. Pediatr. Neonatol. 54, 360–366 (2013).

Lozano, B., Castellote, A. I., Montes, R. & López-Sabater, M. C. Vitamins, fatty acids, and antioxidant capacity stability during storage of freeze-dried human milk. Int. J. Food Sci. Nutr. 65, 703–707 (2014).

How to Cite
Enggar, Paula Klara M. Pengaruh suhu dan lama penyimpanan terhadap antioksidan ASI. Media ilmu kesehatan [Internet]. 2022May28 [cited 2022Jul.5];10(3):204-16. Available from: