Influence of dry rendering dan ultrasonic‐assisted extraction on DPPH radical scavenging activity of red fruit (pandanus conoideus)

Authors

  • Mitsalina Fildzah Arifah Universitas Jenderal Ahmad Yani Yogyakarta
  • Kholif Sholehah Indra Kurniasih Universitas Jenderal Achmad Yani Yogyakarta
  • Endah Kurniawati Universitas Jenderal Ahmad Yani Yogyakarta
  • Mustika Erlinaningrum Badan Pengawas Obat dan Makanan, Unit Manokwari, Papua Barat
  • Zaskia Nora Melanie Universitas Jenderal Achmad Yani Yogyakarta

DOI:

https://doi.org/10.30989/mik.v14i2.1471

Keywords:

Antioxidant, DPPH, Dry Rendering, Red Fruit, UAE

Abstract

Background: Red fruit (Pandanus conoideus) is a source of antioxidant compound components. Extraction methods can affect the antioxidant activity of the red fruit oil (RFO). Dry rendering is often used in conventional oil extraction, but it can potentially degrade antioxidant components. Currently, ultrasonic-assisted extraction (UAE) is a modern, yet underexplored method for oil extraction.
Objective: This study aims to compare the extraction method on the antioxidant activity of RFO.
Methods: The extraction methods included dry rendering and ultrasound-assisted extraction (UAE) using water and n-hexane as solvents. The flavonoid and phenolic contents, percentage yield, and DPPH free radical scavenging antioxidant activity were evaluated in RFO.
Results: The results showed that the % yield with dry rendering was higher than in the UAE. The three extracts showed the presence of flavonoids and phenolics based on FeCl3 and AlCl3 tests. The highest antioxidant activity was in the dry rendering method (0,13% w/v) compared to UAE-water (0,98% w/v) and UAE-n hexane (1,47% w/v). Dry rendering could separate bioactive compounds with the highest % yield and inhibit DPPH free radicals with high temperatures, and without solvents.
Conclusion: The dry rendering was selected as the extraction method, resulting in higher yields and antioxidant compounds in EBM compared to UAE.

Author Biographies

Mitsalina Fildzah Arifah, Universitas Jenderal Ahmad Yani Yogyakarta

Jl. Siliwangi, Ringroad Barat, Gamping, Sleman, Daerah Istimewa Yogyakarta, Indonesia.

Kholif Sholehah Indra Kurniasih, Universitas Jenderal Achmad Yani Yogyakarta

Jl. Siliwangi, Ringroad Barat, Gamping, Sleman, Daerah Istimewa Yogyakarta, Indonesia.

Endah Kurniawati, Universitas Jenderal Ahmad Yani Yogyakarta

Jl. Siliwangi, Ringroad Barat, Gamping, Sleman, Daerah Istimewa Yogyakarta, Indonesia.

Mustika Erlinaningrum, Badan Pengawas Obat dan Makanan, Unit Manokwari, Papua Barat

 Jalan Angkasa Mulyono, Amban, Manokwari, Papua Barat, Indonesia.

Zaskia Nora Melanie, Universitas Jenderal Achmad Yani Yogyakarta

Jl. Siliwangi, Ringroad Barat, Gamping, Sleman, Daerah Istimewa Yogyakarta, Indonesia.

References

1. Palupi IA, Martosupono M. Buah merah: Potensi dan Manfaatnya sebagai Antioksidan. The Journal of Indonesian Medicinal Plant. 2009;2(1):42–8.
2. Letviany Z, Hariyadi P, Purnomo EH. Pengaruh Metode Ekstraksi terhadap Mutu Kimia dan Komposisi Asam Lemak Minyak Buah Merah (Pandanus conoideus). Jurnal Teknologi Industri Pertanian [Internet]. 2014;24(3):209–17. Available from: https://www.researchgate.net/publication/273350504
3. Murtiningrum, Ketaren S, Suprihatin, Kaseno. Ekstraksi Minyak dengan Metode Wet Rendering dari Buah Pandan (Pandanus conoideus L). Jurnal Teknologi Industri Pertanian. 2005;
4. Susanti EP, Rohman A, Setyaningsih W. Dual Response Optimization of Ultrasound-Assisted Oil Extraction from Red Fruit (Pandanus conoideus): Recovery and Total Phenolic Compounds. Agronomy. 2022 Feb 1;12(2).
5. Wang W, Jung J, Tomasino E, Zhao Y. Optimization of solvent and ultrasound-assisted extraction for different anthocyanin rich fruit and their effects on anthocyanin compositions. LWT. 2016 Oct 1;72:229–38.
6. Kumar K, Srivastav S, Sharanagat VS. Ultrasound assisted extraction (UAE) of bioactive compounds from fruit and vegetable processing by-products: A review. Vol. 70, Ultrasonics Sonochemistry. Elsevier B.V.; 2021.
7. Sarungallo ZL, Hariyadi P, Andarwulan N, Purnomo EH. Keragaman Karakteristik Fisik Buah, tanaman dan Rendemen Minyak dari 9 Klon Buah Merah (Pandanus conoideus). Agrikan: Jurnal Agribisnis Perikanan. 2019 Jun 14;12(1):70.
8. Efendi SC, Dwi Anggo A, Wijayanti I. Pengaruh Suhu Ekstraksi Pada Metode Dry Rendering Terhadap Kualitas Minyak Kasar Hati Ikan Manyung (Arius thalassinus). Vol. 2, Jurnal Ilmu dan Teknologi Perikanan. 2020.
9. Erlinaningrum M, Rohman A, Murti AA, Hastuti B. The Effects of Activated Carbon and Bentonite on Physicochemical Characterization of Red Fruit Oil (Pandanus conoideus Lam). Journal of Food and Pharmaceutical Sciences [Internet]. 2024;(2):90–6. Available from: www.journal.ugm.ac.id/
10. Sundari I, Wibowo FR. Identifikasi Senyawa dalam Ekstrak Etanol Biji Buah Merah (Pandanus conoideus). Biofarmasi. 2015;13(2):78–89.
11. Wigati EI, Pratiwi E, Nissa TF, Utami NF. Uji Karakteristik Fitokimia dan Aktivitas Antioksidan Biji Kopi Robusta (Coffea canephora Pierre) dari Bogor, Bandung dan Garut dengan Metode DPPH (1,1-diphenyl-2-picrylhydrazyl). FITOFARMAKA: Jurnal Ilmiah Farmasi. 2019 May 30;8(1):53–9.
12. Sangkala SA, Jura MR, Tangkas IM. Uji Aktivitas Antioksidan Ekstrak Buah Merah (Pandanus baccari L) di Daerah Poso Sulawesi Tengah. JAkad Kim. 2014;3(4):198–205.
13. Molyneux P, Associates M. The use of the stable radical Diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Journal of Sciences and Technology [Internet]. 2004;26(2):211–9. Available from: https://www.researchgate.net/publication/237620105
14. Arifah MF, Irnawati, Ruslin, Nisa K, Windarsih A, Rohman A. The Application of FTIR Spectroscopy and Chemometrics for the Authentication Analysis of Horse Milk. Int J Food Sci. 2022;2022.
15. Rohman A, Ghazali MAIB, Windarsih A, Irnawati, Riyanto S, Yusof FM, et al. Comprehensive Review on Application of FTIR Spectroscopy Coupled with Chemometrics for Authentication Analysis of Fats and Oils in the Food Products. Molecules. 2020;25(22).
16. Windarsih A, Irnawati, Rohman A. The use of FTIR Spectroscopy in Combination with Chemometrics for The Authentication of Milk Fat from Palm Oil. IOP Conf Ser Mater Sci Eng. 2020;980(1).
17. Miller JC, Miller JN. Chemometrics for Analytical. 5th ed. Pearson Education Limited; 2005.
18. Gligor O, Clichici S, Moldovan R, Muntean D, Vlase AM, Nad?? GC, et al. The Effect of Extraction Methods on Phytochemicals and Biological Activities of Green Coffee Beans Extracts. Plants. 2023 Feb 1;12(4).
19. Bligh EG, Dyer WJ. A rapid method of total lipid extraction and purification. Can J Biochem Physiol [Internet]. 1959;37(8):911–7. Available from: https://pubmed.ncbi.nlm.nih.gov/13671378/
20. Firdausia RS, Kurniasih KSI, Diani A. Analisis Potensi Antioksidan Daun Kayu Bulan (Pisonia alba Span.) sebagai Agen Anti Penuaan Dini. Chimica et Natura Acta [Internet]. 2023;11(1):22–8. Available from: http://jurnal.unpad.ac.id/jcena
21. Hamdo HH, Khayata W, Al-Assaf Z. Synergistic Effect of combined some natural and synthetic antioxidants to increase oxidative stability using DPPH test. Article in International Journal of Chem Tech Research [Internet]. 2014;6(4):2539–45. Available from: https://www.researchgate.net/publication/280944538
22. Rohman A, Che Man YB, Riyanto S. Authentication Analysis of Red Fruit (Pandanus conoideus Lam) Oil Using FTIR Spectroscopy in Combination with Chemometrics. Phytochemical Analysis. 2011 Sep 24;22(5):462–7.
23. Zin NBM, Azemin A, Muslim M, Rodi M, Rashid ZM, Mohd KS. Application of FTIR fingerprints Coupled with Chemometric for Comparison of Stingless Bee Propolis from Different Extraction Methods. Malaysian Journal of Fundamental and Applied Sciences Special Issue on International Conference on Agriculture. 2019;350–5.
24. Rohman A, Windarsih A, Riyanto S, Sudjadi, Shuhel Ahmad SA, Rosman AS, et al. Fourier Transform Infrared Spectroscopy Combined with Multivariate Calibrations for the Authentication of Avocado Oil. Int J Food Prop. 2016;19(3):680–7.
25. Satriyanto B, Widjanarko SB. Heat Stability of Red Fruit Extract Color as Potential Source of Natural Pigments. Jurnal Teknologi Pertanian. 13(3).

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Published

2025-08-01

How to Cite

1.
Arifah MF, Kurniasih KSI, Kurniawati E, Erlinaningrum M, Melanie ZN. Influence of dry rendering dan ultrasonic‐assisted extraction on DPPH radical scavenging activity of red fruit (pandanus conoideus). Media ilmu kesehatan [Internet]. 2025 Aug. 1 [cited 2025 Aug. 16];14(2):108-16. Available from: https://ejournal.unjaya.ac.id/index.php/mik/article/view/1471

Issue

Vol. 14 No. 2 (2025): Media Ilmu Kesehatan

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