• Budi Rahayu Stikes Jenderal Achmad Yani Yogyakarta
  • Nurdiana Universitas Brawijaya Malang
  • Siti Candra Windu Baktiyani RS dr. Saiful Anwar Malang


Background : Preeclampsia is a condition where the blood pressure is increasing (≥140/90 mmHg) followed by proteinuria (≥300 mg/24 hour or ≥1+ in stick test) in pregnant women after 20 weeks of pregnancy. Released placenta factor cause the damage in endothelial cells which lead to the excessive increase on pro inflammation factor interleukin 6 (IL-6).

Objective: This study aimed to investigate whether level IL-6 on normal HUVECs with normal HUVECs induced by plasma from preeclamptic patients.

Research Method: True experimental research using posttest only control group approach, conducted in the laboratory with invitro. HUVECs culture was divided into 5, that were normal HUVECs without treatment (K-); HUVECs preeclampsia model (K+). Measurement on the levels of IL-6 used ELISA (Enzyme-Linked Immunosorbent Assay).

Result: Independent sample t-test showed that there were significant differences (p=0.000<α) on the average of IL-6 levels between the negative control group (1.64±0.12 pg/ml) and the positive control group (31.64±11.69 pg/ml).

Conclusion: Adding the patient’s preeclampsia plasma into normal HUVECs can increase levels of IL-6.

Keyword: Patient’s preeclampsia plasma, HUVECs, and IL-6.

Author Biographies

Budi Rahayu, Stikes Jenderal Achmad Yani Yogyakarta

Jl. Ringroad Barat Ambarketawang Gamping Sleman Yogyakarta 0274 434 2000

Nurdiana, Universitas Brawijaya Malang

Laboratorium Farmakologi Fakultas Kedokteran JL. Veteran, Malang, East Java, 65145, Ketawanggede, Lowokwaru, Malang City, East Java 65145

Siti Candra Windu Baktiyani, RS dr. Saiful Anwar Malang

Laboratorium Obstetri Dan Ginekologi Fakultas Kedokteran Brawijaya Malang Jl. Jaksa Agung Suprapto No.2, Klojen, Kec. Klojen, Kota Malang, Jawa Timur 65111


1. Cunningham FG, Mac Donald PC, Gant NF, Leveno KJ, Gilstrap LC, Hakins GD, et al. Williams obstetrics. 23th ed. London: Prentice-Hall International; 2010.
2. Wang A, Rana S, Karumanchi AS. Preeclampsia: The role of angiogenic factors in its pathogenesis. Physiology. 2009; 24:147-58.
3. Borzychowski AM, Sargent IL, Redman CW. Inflammation and preeklampsia. Semin Fetal Neonatal Med. 2006; 11(5): 309-16.
4. Gilbert JS, Ryan MJ, La marca BB, Sedeek M, Murphy SR, Granger JP. Pathophysiology of hypertension during preeclampsia: Linking placental ischemia with endothelial dysfunction. Am J Obstet Gynecol. 2008; 294:541-50.
5. Redman CWG, Sargent IL, Immunology of pre-eclampsia. American Journal of Reproductive Immunology. 2010; 63:534- 43.
6. Dharma R, Wibowo N, Raranta PTH. Disfungsi endotel pada preeklampsia. Makara Kesehatan. 2005; 9(2):63-69.
7. Susianto IA, Suharsono, Hadijono S. Kadar TNF-α, IL-6 dan trofoblas pada preeklampsia – Eklampsia. Medika Media Indonesia. 2009; 43(4):166-73.
8. Takacs P, Green KL, Nikaeo A, Kauma SW. Increased vascular endothelial cell production of interleukin-6 in severe preeclampsia. Am J Obstet Gynecol. 2003; 188:740-4.
9. Gill-Villa AM, Norling LV, Serhan CN, Cordero D, Rojas M, Cadavid A. Aspirin triggered-lipoxin A4 reduced the adhesion of human polymorphonuclear neutrophils to endothelial cells initiated by preeclamptic plasma. Prostaglandins, Leukotrienes and Essential Fatty Acids (PLEFA). 2012; 87:127-34.
10. Min J, Kim Y, Kim S, Kwon M, Kong Y, Hwang IK, Won MH, Rho J, Kwon Y. TNF-related activation induced cytokine enchaces leukocyte adhesiveness: induction of ICAM-1 and VCAM-1 via TNF receptor-associated factor and protein kinase C-dependent NF-kB activation in endothelial cells. J Immunol. 2005; 175:531-40.
11. Anwar AD, Achmad TH, Sukandar H. Polimorfisme C1167T gen reseptor tipe IItransforming growth factor-β, kadar soluble endoglin, dan vascular cell adhesion molecule-1 pada preeklampsia. MKB. 2010; 42(3):115-22.
12. Ramma W, Ahmed A. Is inflammation the cause of pre-eclampsia. Biochemical Society Transaction. 2011; 39:1619-27.
13. Taylor, RN, Davidge, ST, Roberts JM. Endothelial cell dysfunction and oxidative stress. In Lindhemer MD, Roberts JM, Cunningham FG, editors. Chesley’s Hypertensive Disorders in Pregnancy. 3rd ed. Elsevier
14. Faas MM, Schuiling GA, Linton EA, Sargent IL, Redman CWG. Activation of peripheral leukocytes in rat pregnancy and experimental preeclampsia . Am J Obstet Gynecol. 2000; 182:351
15. Faisel K, Galarraga , Belch, JJF. The role of endothelial function and its assesment in rheumatoid arthritis. Nature Reviews Rheumatology. 2010; 6:253-61.
16. Wang Y, Lewis FD, Gu Y, Zhao S, Groome JL. Elevated maternal soluble gp130 and il-6 levels and reduces gp 130 and socs-3 exspressions in women complicated with preeclampsia. Hypertension American heart Association. 2010; 57:336-42.
How to Cite
Rahayu B, Nurdiana, Windu Baktiyani SC. PERBEDAAN KADAR IL-6 PADA HUVECs NORMAL DENGAN HUVECs NORMAL YANG DIPAPAR PLASMA HAMIL PENDERITA PREEKLAMPSIA. Media ilmu kesehatan [Internet]. 2019Nov.8 [cited 2024Jul.16];4(3):198-02. Available from: