PERBEDAAN KADAR IL-6 PADA HUVECs NORMAL DENGAN HUVECs NORMAL YANG DIPAPAR PLASMA HAMIL PENDERITA PREEKLAMPSIA

  • Budi Rahayu Stikes Jenderal Achmad Yani Yogyakarta
  • Nurdiana Universitas Brawijaya Malang
  • Siti Candra Windu Baktiyani RS dr. Saiful Anwar Malang

Abstract

Background : Preeclampsia is a condition where the blood pressure is increasing (≥140/90 mmHg) followed by proteinuria (≥300 mg/24 hour or ≥1+ in stick test) in pregnant women after 20 weeks of pregnancy. Released placenta factor cause the damage in endothelial cells which lead to the excessive increase on pro inflammation factor interleukin 6 (IL-6).

Objective: This study aimed to investigate whether level IL-6 on normal HUVECs with normal HUVECs induced by plasma from preeclamptic patients.

Research Method: True experimental research using posttest only control group approach, conducted in the laboratory with invitro. HUVECs culture was divided into 5, that were normal HUVECs without treatment (K-); HUVECs preeclampsia model (K+). Measurement on the levels of IL-6 used ELISA (Enzyme-Linked Immunosorbent Assay).

Result: Independent sample t-test showed that there were significant differences (p=0.000<α) on the average of IL-6 levels between the negative control group (1.64±0.12 pg/ml) and the positive control group (31.64±11.69 pg/ml).

Conclusion: Adding the patient’s preeclampsia plasma into normal HUVECs can increase levels of IL-6.

Keyword: Patient’s preeclampsia plasma, HUVECs, and IL-6.

Author Biographies

Budi Rahayu, Stikes Jenderal Achmad Yani Yogyakarta

Jl. Ringroad Barat Ambarketawang Gamping Sleman Yogyakarta 0274 434 2000

Nurdiana, Universitas Brawijaya Malang

Laboratorium Farmakologi Fakultas Kedokteran JL. Veteran, Malang, East Java, 65145, Ketawanggede, Lowokwaru, Malang City, East Java 65145

Siti Candra Windu Baktiyani, RS dr. Saiful Anwar Malang

Laboratorium Obstetri Dan Ginekologi Fakultas Kedokteran Brawijaya Malang Jl. Jaksa Agung Suprapto No.2, Klojen, Kec. Klojen, Kota Malang, Jawa Timur 65111

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Published
2019-11-08
How to Cite
1.
Rahayu B, Nurdiana, Windu Baktiyani SC. PERBEDAAN KADAR IL-6 PADA HUVECs NORMAL DENGAN HUVECs NORMAL YANG DIPAPAR PLASMA HAMIL PENDERITA PREEKLAMPSIA. Media ilmu kesehatan [Internet]. 2019Nov.8 [cited 2024Jul.16];4(3):198-02. Available from: https://ejournal.unjaya.ac.id/index.php/mik/article/view/133