The effect of maceration duration on the total flavonoids content of kaempferia parviflora wall. ex baker ethanol extract
DOI:
https://doi.org/10.30989/mik.v13i1.1300Keywords:
flavonoids, kaempferia parviflora, maceration, uv-vis spectrophotometricAbstract
Background: Kaempferia parviflora (Black ginger) has phytochemical compounds such as flavonoids, terpenoids, essential oils, and phenols that have the potential to treat various diseases. To maximize the total extracted flavonoids needs to optimize the extraction duration.
Objective: Knowing the optimum maceration duration on the total flavonoid content using the UV-VIS Spectrophotometric method on black ginger ethanol extract.
Method: Black ginger is extracted using the maceration method with ethanol (70%) solvent in maceration duration of 12, 24, 36,48, and 60 hours. The extract will be tested qualitatively and quantitative to determine the total levels of flavonoids using the UV-Vis Spectrophotometric method.
Result: Qualitative test of black ginger ethanol extract gave positive results for flavonoids, phenolics, alkaloids, steroids, and tannins. The total level of flavonoids in black ginger ethanol extract was 7,8988 ? 0,1223 mgEQ/g (12 hours); 8,5705 ? 0,1529 mgEQ/g (24 hours); 9,8102 ? 0,0416 mgEQ/g (36 hours); 10,6161 ? 0,0768 mgEQ/g (48 hours); 7,2594 ? 0,1244 mgEQ/g (60 hours). The statistical analysis indicates a significant difference (p < 0,05) in the total flavonoid content at each maceration time.
Conclusion: The duration of maceration time significantly affects the total flavonoid content produced with the optimum maceration time results at 48 hours
References
Tim Bina Karya Tani, 2009. Budidaya Tanaman Jahe. Yrama Widya. Bandung.
Saokaew, S., Wilairat, P., Raktanyakan, P., Dilokthornsakul, P., Dhippayom, T., Kongkaew, C., Sruamsiri, R., Chuthaputti, A., & Chaiyakunapruk, N. (2017). Clinical Effects of Krachaidum (Kaempferia parviflora): A Systematic Review. Journal of Evidence-Based Complementary and Alternative Medicine, 22(3), 413–428. https://doi.org/10.1177/2156587216669628
Sornpet, B., Potha, T., Tragoolpua, Y., & Pringproa, K. (2017). Antiviral activity of five Asian medicinal pant crude extracts against highly pathogenic H5N1 avian influenza virus. Asian Pacific Journal of Tropical Medicine, 10(9), 871–876. https://doi.org/10.1016/j.apjtm.2017.08.010
Ab Rahman, Z., Abd Shukor, S., Abbas, H., A. L. Machap, C., Suhaimi Bin Alias, M., Mirad, R., Sofiyanand, S., & Nazreena Othman, A. (2018). Optimization of Extraction Conditions for Total Phenolics and Total Flavonoids from <i>Kaempferia parviflora</i> Rhizomes. Advances in Bioscience and Biotechnology, 09(05), 205–214. https://doi.org/10.4236/abb.2018.95014
Chaisuwan, V., Dajanta, K., & Srikaeo, K. (2022). Effects of extraction methods on antioxidants and methoxyflavones of Kaempferia parviflora. Food Research, 6(3), 374–381. https://doi.org/10.26656/fr.2017.6(3).408
Voight, R. 1995. Buku Pelajaran Teknologi Farmasi. Edisi Kelima. Yogyakarta: Gadjah Mada University Press.
Yeo, Y. L., Chia, Y. Y., Lee, C. H., Sheng Sow, H., & Sum Yap, W. (2014). Effectiveness of Maceration Periods with Different Extraction Solvents on in-vitro Antimicrobial Activity from Fruit of Momordica charantia L. Journal of Applied Pharmaceutical Science, 4(10), 16–23. https://doi.org/10.7324/japs.2014.401004
Agustina, W., & Handayani, D. (2017). Skrining Fitokimia dan Aktivitas Antioksidan Beberapa Fraksi Dari Kulit Batang Jarak (Ricinus communis L.). Jurnal Pendidikan Dan Ilmu Kimia, 1(2), 117–122.
Manongko, P. S., Sangi, M. S., & Momuat, L. I. (2020). Phytochemical Compound Test and Antioxidant Activity of Broken Bone Plants (Euphorbia tirucalli L.). Jurnal MIPA, 9(2), 64.
Fitriyani, A., Winarti, L., Muslichah, S., & Nuri, D. (2021). Uji antiinflamasi ekstrak metanol daun sirih merah (piper crocatum ruiz & pav ) pada tikus putih anti-inflammatory activityy of piper crocatum ruiz & pav. Leaves metanolic extract in rats. Majalah Obat Tradisional, 16(1), 2011.
Sari, A. N., & Asri, M. T. (2022). Aktivitas Antibakteri Ekstrak Kulit Jeruk Nipis (Citrus aurantifolia) terhadap Pertumbuhan Bakteri Shigella dysenteriae. LenteraBio, 11(3), 441–448.
Antarti A. N. and Lisnasari R. Uji Aktivitas Antioksidan Ektrak Ethanol Daun Family Solanum Menggunakan Metode Reduksi Radikal Bebas DPPH. JPSCR : Journal of Pharmaceutical Science and Clinical Research, vol. 3, no. 2, p. 62, Oct. 2018, doi:10.20961/jpscr.v3i2.15378.
Pertiwi, F. D., Rezaldi, F., & Puspitasari, R. (2022). Uji Aktivitas Antibakteri Ekstrak Etanol Bunga Telang (Clitoria ternatea L.) Terhadap Bakteri Staphylococcus epidermidis. Biosaintropis (Bioscience-Tropic), 7(2), 57–68.
Maharadingga, M., Pahriyani, A., & Arista, D. (2021). Uji Aktivitas Ekstrak Etanol 70% Daun Ketapang (Terminalia catappa L.) Pada Hamster Syrian Jantan Hiperglikemia Dan Hiperkolesterolemia Dengan Parameter Pengukuran Kolesterol Total Dan LDL. Lumbung Farmasi: Jurnal Ilmu Kefarmasian, 2(2), 80.
Syamsul, E. S., Amanda, N. A., & Lestari, D. (2020). Perbandingan Ekstrak Lamur Aquilaria malaccensis Dengan Metode Maserasi dan Refluks. Jurnal Riset Kefarmasian Indonesia, 2(2), 97–104.
Ramadhan, S., Ramadhan, A., Laenggeng, A. H., & Kundera, I. N. (2022). Pengaruh Kombinasi Ekstrak Daun Pare (Momordica charantia L.) dan Kunyit (Curcuma longa) Terhadap Kadar Kreatinin Pada Tikus (Rattus norvegicus) yang Diinduksi CCl4. Journal of Biology Science and Education, 9(2), 780–785. https://doi.org/10.22487/jbse.v9i2.1733
Kusumawardani, Z., Kusnadi, & Santoso, J. (2020). Pengaruh Konsentrasi Etanol 70%, 90%, 95% Terhadap Kandungan Flavonoid Pada Ekstrak Daun Katuk (Sauropus androgynus (L.). Jurnal Ilmiah Farmasi, 10(10), 1–5.
Wijayanti , Ni Putu Ayu Dewi, Dewi, L.P.M.K., Astuti, K.W AND Fitri, N.P.E. (2016). Optimasi Waktu Maserasi untuk Manggis (Garcinia mangostana L.) Rind Menggunakan Pelarut Etil Asetat. Jurnal Farmasi Dan Ilmu Kefarmasian Indonesia, 3(1), 12–16.
Fernanda, T. (2019). Aplikasi Pemanfaatan Daun Pepaya (Carica Papaya) Sebagai Biolarvasida Terhadap Larva Aedes Aegypti (N. Hariyati (ed.); 1st ed.). Granti.
Fatmawati, S. (2019). Pengaruh Perbedaan Metode Ekstraksi Maserasi dan Perkolasi terhadap Uji Aktivitas Antioksidan Ekstrak Kulit Buah Naga Merah (Hylocereus polyrhizus). Jurnal Industri Pertanian, 2(1), 95–102.
Downloads
Published
How to Cite
Issue
Section
License
Articles received and published by the Media Ilmu Kesehatan are by the publication, the copyright of the article is fully transferred to the Media Ilmu Kesehatan. All operational forms such as printing, publication, and distribution of hard file journals are carried out by the Media Ilmu Kesehatan. Articles that have finished the review process and have been declared accepted by the journal manager or editor will be asked to fill out a statement of submission of copyright by the journal secretary to the main author or correspondent author. The statement of transfer of copyright is signed with a seal and sent via email to journalmik2018@gmail.com and contacted the admin of the journal to be followed up on archiving. Journal managers and editors have the right to edit the manuscript according to the provisions of the writing rules in the Media Ilmu Kesehatan.
Articles that have been declared accepted either online through the author's account on the OJS website https://ejournal.unjaya.ac.id/index.php/mik or a letter of receipt of the article (LOA), as well as those that have been published on OJS are not allowed to be published in other journals, or proceedings. The number of authors with more than one and as the main author or designated as the correspondent writer must have coordinated with members of the research team. The order of the authors submitted in the article as the author of one, two, three and so on cannot be changed when the article is published unless an error occurs in the technical operation of the journal.
