Interaction Affinity Between Flavonoids Against Cytokines Interleukin 12 in Diabetes Mellitus
Background: Interleukin 12 can destroy insulin-producing cells, suppresses IL4 production, and can stimulate the formation of Thelper1. Quercetin is a flavonoid suitable to be chosen as the lead compound for development of safe anti-diabetic agent because in addition to its anti-diabetic effect, quercetin shows also protective effect in pancreas track.
Objective: This research aims to study the docking models of certain flavonoids and to predict the quercetin and triterpene derivatives inhibition activity on Interleukin12.
Method: The molecular docking method was used using the PyRx program with the Autodock Vina menu.
Results: IL-12 bond affinity with Dextromethoorpene results -7 kcal/mol as the highest affinity value energy while the lowest energy is -6.1 kcal/mol, IL-12 bond affinity value with Quercetin with the highest affinity value energy is -9 kcal/mol, and the lowest energy is -7.8kcal/mol. The affinity value IL-12 bond and triterpene with the highest affinity value is energy -7 .9 kcal/mol, and the lowest energy is -7.4 kcal/mol
Conclusion: Quercetin, has hydrogen bonds and hydrophobic bonds, where the ligand of the Quercetin compound is the ligand that has the ability to form the best interactions and bonds with IL-12 receptors (4OG9)
Lackie, JA. Dictionary of Biomedicine. Oxford University Press. England; 2010.
Gaffen SL. "Struktur dan sinyal keluarga IL-17 reseptor" . Nat. Rev Immunol 9 (8): 556-67; 2009.
Wuryandari MRE, Widodo N, Widjajanto E, Jatmiko YD, Rifa’i M. Red Moringa oleifera leaf fermentation extract protecting Hepatotoxicity in Balb/C mice injected with Salmonella typhi through Nrf-2, HO-1, and SOD- 2 signaling pathways. Res J Pharm Technol. 13:1e6; 2020.
American Diabetes Association. Diagnosis and classification of diabetes mellitus. Diabetes Care. 33(Suppl 1): S6; 2010.
Hartati Tuna, Wuryandari MRE, Moh Sofi. Hubungan Kadar Glukosa Darah dengan Glomerular Filtration Rate (GFR) Pada Pasien Diabetes Melitus dengan Obesitas Di RSU Daha Husada Kediri. Jurnal Sintesis Vol 3(2), pp: 62-67; 2022
Leonard WJ. Type 1 cytokines and interferons and their receptor. In: Paul WE, Editor. Fundamental immunology. Fild edition. Philadelphia. Lippincott William and Wilkins p. 701-35; 2003
Longo, D., Fauci, A., Kasper, D., & Hauser, S. Harrison's Principles of internal medicine. Edisi ke-18. New York: McGraw-Hill Professional; 2011.
Baratawidjaja K dan Rengganis I, “Sitokin”, Dalam Imunologi Dasar, edisi 10, Badan Penerbit FKUI, Jakarta, hala 217-32; 2014.
Wegner M, Winiarska H, Kozlowska T, Dworacka M, “IL-12 Serum Levels in Patients with Type 2 Diabetes Treated with Sulphonylureas”, Cytokine, Elsevier, p.312-6; 2008.
Yong K, Dogra G, Bouville N, Chan D, Adams L, Ching H, Lim E, “Interleukin-12 is Associated With Arterial Stiffness in Healthy Individuals, American Journal of Hypertension, 26, p. 159-62; 2013.
Wuryandari MRE, Mochammad Fitri Atho'illah , Rizky Dzariyani Laili , Siti Fatmawati , Nashi Widodo , Edi Widjajanto , Muhaimin Rifa’I, Lactobacillus plantarum FNCC 0137 fermented red Moringa oleifera exhibits protective effects in mice challenged with Salmonella typhi via TLR3/TLR4 inhibition and down-regulation of proinflammatory cytokines. J.Ayuverda 13(2):100531; 2022
Fatmawati S, Laili RD, Wuryandari MRE, Martati E, Widyaningsih TD, Rifa’iM. Fermented ethanolic extract of Moringa oleifera leaves with Lactobacillus plantarum FNCC 0137 as immunomodulators on Salmonella typhi infected mice. Res J Pharm Technol. 13(12):1e6; 2020.
Gupta, R., Mathur, M., Bajaj, V. K., Katariya, P., Yadav, S., Kamal, R., & Gupta, R. S. Evaluation of antidiabetic and antioxidant activity of Moringa oleifera in experimental diabetes. Journal of diabetes. 4(2):164-71; 2012.
Gupta, R., & Gupta, R. S. Effect of Pterocarpus marsupium in streptozotocin-induced hyperglycemic state in rats: comparison with glibenclamide. Diabetologia Croatica. 38(2):39-45; 2009
Oda, A., Okayasu, M., Kamiyama, Y., Yoshida, T.,Takahashi, O., and Matsuzaki, H., Bull. Chem.Soc. Jpn., 80, 10, 1920-1925; 2007.
K. E. Saputri, N. Fakhmi, E. Kusumaningtyas, D. Priyatama, and B. Santoso. Docking Molekular Potensi Anti Diabetes Melitus Tipe 2 Turunan Zerumbon Sebagai Inhibitor Aldosa Reduktase Dengan Autodock-Vina. Chim. Nat. Acta. vol. 4, no. 1, p. 16; 2016.
W. Forli, S. Halliday, R. Belew, and A.
Olson. 2002. AutoDock Version 4.2,”
Citeseer, pp. 1–66. [Online]. Available:http://citeseerx.ist.psu.edu/viewdoc/downlo ad?. doi=10.1.1.363.3063&rep=rep1&type pdf.
G. Syahputra, L. Ambarsari, and T. Sumaryada. Simulasi Docking Kurkumin Enol , Bismetoksikurkumin Dan Analognya Sebagai Inhibitor Enzim12-Lipoksigenase,” J. Biofisika. vol. 10, no. 1, pp. 55–67; 2014.
Guo xiu Zu , Keyun Sun , Ling Li , Xiuli Zu , Tao Han3 & Hailiang Huang Mechanism of quercetin
therapeutic targets for Alzheimer disease and type 2 diabetes mellitus. Scientific report. 11:22959; 21
Diyan Sakti Purwanto, Hari Susanti, Nining Sugihartini. Docking Molekuler Potensi Anti Inflamasi quercetin daun kelor (Moringa oleifera L.) dengan Autodock-Vina. Jurnal Manusia dan Kesehatan; 2021.
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