• Serafica Btari Christiyani Kusumaningrum tikes Guna Bangsa Yogyakarta
  • Wiwit Eepvianti STIKES Guna Bangsa Yogyakarta


Background: Bacterial contamination in thrombocyte concentrate (TC) blood product becomes a serious problem since it can cause clinical risks from mild symptom to death. TC storage condition at 22-25oC with agitation is good condition for bacterial growth. Source of bacterial contamination can come from donor bacteremia, not aseptically from taking and processing blood product. In a previous study, showed there were positive gram bacteria as bacterial contamination in TC.

Objective: This objective aims to determine bacterial contaminants in TC blood products.  

Methods: Total of 3 TC bag samples were collected from UDD PMI Kabupaten Sleman. Each sample was inoculated to nutrient agar media and it incubated for 48 jam at 37oC. Observation of bacterial colony growth is carried out after incubation time is over.

Results: There was no bacterial growth in the media so that there was no bacterial contamination in TC. It showed that the procedures, processing, and storage of TC products in PMI Kabupaten Sleman were aseptic. Besides that, TC donors also did not suffer from bacteremia so there was bacterial contamination in TC.

Conclusion: In this study, bacterial contamination in blood product TC was not found since there was no bacterial growth in media. 

Bacterial contamination, blood products, blood bank, thrombocyte concentrate.


Download data is not yet available.

Author Biographies

Serafica Btari Christiyani Kusumaningrum, tikes Guna Bangsa Yogyakarta

Stikes Guna Bangsa Yogyakarta, Jalan Ringroad Utara Condong Catur Sleman Yogyakarta, Indonesia

Wiwit Eepvianti, STIKES Guna Bangsa Yogyakarta

Stikes Guna Bangsa Yogyakarta, Jalan Ringroad Utara Condong Catur Sleman Yogyakarta, Indonesia


1. Booth C, Allard S. Blood transfusion. Medicine (Baltimore) [Internet]. Elsevier; 2017 Apr 1;45(4):244–50. Available from:
2. Delaney M, Wendel S, Bercovitz RS, Cid J, Cohn C, Dunbar NM, et al. Transfusion reactions: prevention, diagnosis, and treatment. Lancet (London, England). England; 2016 Dec;388(10061):2825–36.
3. Sudiana IK. Analisis Produk Darah Thrombocyte Concentrate di Palang Merah Indonesia Surabaya ( Analysis of Blood Product Trombocyte Concentrate in Red Cross Blood Donor Unit Surabaya ). 2012;14(3).
4. Farzad BB, Farshad B, Zahra B, Nahid A, Mahsa KB. Bacterial contamination of platelet products in the Blood Transfusion Center of Isfahan, Iran. GMS Hyg Infect Control. 2016;11:Doc23.
5. Muller TH, Mohr H, Montag T. Methods for the detection of bacterial contamination in blood products. Clin Chem Lab Med. Germany; 2008;46(7):933–46.
6. Ketter P, Arulanandam B, Cap AP. Platelets Feeding Bacteria with Lactate during Room Temperature Storage: Mitigated By Refrigeration. Blood [Internet]. 2017 Dec 7;130(Suppl 1):2407 LP-2407. Available from:
7. Velashjerdi Z, Deyhim MR, Razjou F, Eydi A. Effect of L-carnitine on platelet bacterial contamination and platelet metabolism during 5 days of storage of platelet concentrates. Blood-Journal 2019 Feb 1;15(1):1–11. Available from:
8. He Z, Che J, Wang Q, Chen S, Chen Q, Yu L, et al. Bacterial contamination of platelet products in Dongguan Blood Center , Guangdong Province of China. 2018;(4):1–5.
9. Horth RZ, Jones JM, Kim JJ, Lopansri BK, Ilstrup SJ, Fridey J, et al. Fatal Sepsis Associated with Bacterial Contamination of Platelets — Utah and California , August 2017. 2018;67(25):718–22.
10. Levy JH, Neal MD, Herman JH. Bacterial contamination of platelets for transfusion: strategies for prevention. Crit Care [Internet]. 2018;22(1):271. Available from:
11. Dickson M, Dinesh D. THE NEW ZEALAND. 2013;126(1374):12–21.
12. Astuti D, Maharani EA. Identifikasi bakteri yang mengontaminasi konsentrat trombosit. :61–7.
13. kemenkes-nomor-91-tahun-2015-tahun-2016 @ [Internet]. Available from:
14. Bolarinwa RA, Aboderin OA, Odetoyin BW, Adegunloye AB. Bacterial contamination of blood and blood components in a tertiary hospital setting in Nigeria. 1996;1–6.
15. Hassall O, Maitland K, Pole L, Mwarumba S, Denje D, Wambua K. T r a n s f u s i o n c o m p l i c at i o n s. 2009;49(December):2594–8.
16. Störmer M, Vollmer T. Diagnostic methods for platelet bacteria screening: current status and developments. Transfus Med Hemother [Internet]. 2013/12/30. S. Karger Verlag für Medizin und Naturwissenschaften GmbH; 2014 Feb;41(1):19–27. Available from:
How to Cite
Kusumaningrum S, Eepvianti W. ANALISIS PRODUK DARAH: KONTAMINASI BAKTERI PADA THROMBOCYTE CONCENTRATE [Internet]. Media Ilmu Kesehatan [Internet]. 31Jan.2019 [cited 20Jun.2019];7(3):244-9. Available from: