The Effect of Extraction Method on Total Phenolic Contents of Hedyotis corymbosa (L.) Lam.

Abstract

Background: Hedyotis corymbosa (L.) Lam. is an Indonesian herbal plant that has benefits as an anticancer, antibacterial, and antioxidant due to the content of secondary metabolite compounds, one of which is phenolic. This activity is influenced by the levels of active compounds contained in it. The levels of active compounds such as phenolics in an extract can be influenced by the extraction method.

Objective: To determine the effect of extraction methods on the total phenolic content of H. corymbosa

Research methods: H. corymbosa was extracted using maceration, soxhletation, and Ultrasound-Assisted Extraction (UAE) methods with 70% ethanol solvent (1:10 w/v). Each extract obtained was tested for total phenolic content using the UV-Vis Spectrophotometry method. The total phenolic content data obtained were then analyzed statistically using Statistical Product and Service Solutions with the One-Way ANOVA and Posy Hoc Tukey tests with a confidence level of 95%.

Results: The total phenolic content of H. corymbosa extract using the maceration extraction method was 53.585 ± 0.910 mg GAE/g, the soxhletation method was 67.827 ± 1.105 mg GAE/g, and the UAE method was 59.615 ± 1.421 mg GAE/g. Based on statistical analysis using the SPPS One-Way Anova test, there was a significant difference (p<0.05) in total phenolic content between extraction methods.

Conclusion: Differences in extraction methods significantly affect the total phenolic content of H. corymbosa extract. The highest total phenolic content was produced using the soxhletation extraction method, followed by the UAE method and the maceration method.

Keywords : Hedyotis corymbosa (L.) Lam.; Extraction Method; Total Phenolic Content; the UV-Vis Spectrophotometry